CAPRIN1 Monoclonal antibody

CAPRIN1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

6B10F11

Reviews (1)

Cat no : 66352-1-Ig

Synonyms

GPI-anchored membrane protein 1, Cytoplasmic activation- and proliferation-associated protein 1, Cell cycle-associated protein 1, Caprin-1, caprin 1

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Validation Data Gallery

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  WB analysis using 66352-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66352-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66352-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66352-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66352-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66352-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66352-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CAPRIN1 antibody (66352-1-Ig, Clone: 6B10F11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 66352-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 66352-1-Ig(CAPRIN1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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  FC experiment of NIH/3T3 using 66352-1-Ig

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human CAPRIN1 (66352-1-Ig, Clone:6B10F11) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue, human prostate cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: sodium arsenite treated HeLa cells, HeLa cells
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IP: See 1 publications below

Product Information

66352-1-Ig targets CAPRIN1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CAPRIN1 fusion protein Ag7413
• Full Name: cell cycle associated protein 1
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC001731
• Gene Symbol: CAPRIN1
• Gene ID (NCBI): 4076
• RRID: AB_2881732
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAPRIN1(Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.

Publications

Species	Application	Title
human	WB	J Cell Biol; Identification of small molecule inhibitors of G3BP-driven stress granule formation; Authors - Brian D Freibaum
mouse	WB,IP	Cell Rep; Alterations of ceramide synthesis induce PD-L1 internalization and signaling to regulate tumor metastasis and immunotherapy response; Authors - Wyatt Wofford

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Memg (Verified Customer) (01-10-2020)

using caprin1 antibody can't get signal.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:20000

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C