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C5AR1/CD88 Recombinant antibody, PBS Only

C5AR1/CD88 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241712G8

Cat no : 84336-6-PBS

Synonyms

C5AR, C5AR1, C5a-R, C5a R, C5a anaphylatoxin chemotactic receptor 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C5AR1/CD88 Recombinant antibody (84336-6-RR)

    WB analysis using 84336-6-RR (same clone as 84336-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84336-6-RR (C5AR1/CD88 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84336-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using C5AR1/CD88 Recombinant antibody (84336-6-RR)

    IF Staining of THP-1 using 84336-6-RR (same clone as 84336-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using C5AR1/CD88 antibody (84336-6-RR, Clone: 241712G8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84336-6-PBS in a different storage buffer formulation.

Product Information

84336-6-PBS targets C5AR1/CD88 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein
Full Name complement component 5a receptor 1
Calculated Molecular Weight39kDa
GenBank Accession NumberNM_001736.4
Gene Symbol C5aR
Gene ID (NCBI) 728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

C5aR, also named CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production. The deduced sequence of C5aR contains 350 amino acids, giving a calculated molecular weight of 39 kDa. C5aR has an N-linked glycosylation site in the N-terminal extracellular domain. The apparent molecular weight of C5aR is about 40-52 kDa (PMID: 1847994; 9136907; 9136907; 2007135).