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C5AR1/CD88 Recombinant antibody

C5AR1/CD88 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241712G8

Cat no : 84336-6-RR

Synonyms

C5AR, C5AR1, C5a-R, C5a R, C5a anaphylatoxin chemotactic receptor 1

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using C5AR1/CD88 Recombinant antibody (84336-6-RR)

    IF Staining of THP-1 using 84336-6-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using C5AR1/CD88 antibody (84336-6-RR, Clone: 241712G8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84336-6-RR

    Affinity and Kinetic Characterization of 84336-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84336-6-RR against Human C5AR1/CD88 were performed. The affinity constant is 3.63 nM.

Tested Applications

Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84336-6-RR targets C5AR1/CD88 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein
Full Name complement component 5a receptor 1
Calculated Molecular Weight39kDa
GenBank Accession NumberNM_001736.4
Gene Symbol C5aR
Gene ID (NCBI) 728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C5aR, also named CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production. The deduced sequence of C5aR contains 350 amino acids, giving a calculated molecular weight of 39 kDa. C5aR has an N-linked glycosylation site in the N-terminal extracellular domain. The apparent molecular weight of C5aR is about 40-52 kDa (PMID: 1847994; 9136907; 9136907; 2007135).

Protocols

Product Specific Protocols
IF protocol for C5AR1/CD88 antibody 84336-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols