C5AR1/CD88 Recombinant antibody

C5AR1/CD88 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84336-6-RR
Clone No.241712G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

C5AR, C5AR1, C5a-R, C5a R, C5a anaphylatoxin chemotactic receptor 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inU-937 cells, U-87 MG cells
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84336-6-RR targets C5AR1/CD88 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein Predict reactive species
Full Name complement component 5a receptor 1
Calculated Molecular Weight39kDa
GenBank Accession NumberNM_001736.4
Gene Symbol C5aR
Gene ID (NCBI) 728
RRIDAB_3671877
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP21730
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C5aR, also named CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production. The deduced sequence of C5aR contains 350 amino acids, giving a calculated molecular weight of 39 kDa. C5aR has an N-linked glycosylation site in the N-terminal extracellular domain. The apparent molecular weight of C5aR is about 40-52 kDa (PMID: 1847994; 9136907; 9136907; 2007135).

Protocols

Product Specific Protocols
WB protocol for C5AR1/CD88 antibody 84336-6-RRDownload protocol
IF protocol for C5AR1/CD88 antibody 84336-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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