CoraLite® Plus 647 Anti-Human BTLA/CD272 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using CL647-98096

  1x10^6 human PBMCs were surface stained with PE Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (PE-65569, Clone: OKT3), and 5 ul CoraLite® Plus 647 Anti-Human BTLA/CD272 Rabbit RecAb (CL647-98096, Clone: 241318H5) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL647-98096

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human BTLA/CD272 Rabbit RecAb (CL647-98096, Clone:241318H5)(red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

BTLA/CD272 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-98096

Clone No.241318H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

BTLA, B- and T-lymphocyte-associated protein, B- and T-lymphocyte attenuator, B and T lymphocyte associated, B and T lymphocyte attenuator

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is N/A per 10^6 cells in a 100 µl suspension or N/A per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98096 targets BTLA/CD272 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: B and T lymphocyte associated
• Calculated Molecular Weight: 289 aa, 33 kDa
• GenBank Accession Number: BC107091
• Gene Symbol: BTLA
• Gene ID (NCBI): 151888
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7Z6A9
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

BTLA ( B and T lymphocyte attenuator), also known as CD272, is a member of the CD28 superfamily and is a type I membrane glycoprotein identified as an inhibitory receptor (PMID: 33859648). BTLA is extensively expressed in lymph nodes, thymus, and spleen, with little or no expression in organs such as the heart, kidney, brain, and liver (PMID: 33859648). Among immune cells, BTLA is primarily expressed in B and T cells, with higher expression in B cells compared to T cells in the mouse spleen (PMID: 33859648). BTLA plays a crucial role in regulating stimulatory and inhibitory signals in immune responses.