Anti-Human BTLA/CD272 Rabbit Recombinant Antibody

BTLA/CD272 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241318H5

Cat no : 98096-1-RR

Synonyms

BTLA, B- and T-lymphocyte-associated protein, B- and T-lymphocyte attenuator, B and T lymphocyte associated, B and T lymphocyte attenuator

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Validation Data Gallery

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  FC experiment of human PBMCs using 98096-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human BTLA/CD272 Rabbit Recombinant Antibody (98096-1-RR, Clone: 241318H5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (APC-65559, Clone: UCHT4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98096-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human BTLA/CD272 Rabbit Recombinant Antibody (98096-1-RR, Clone: 241318H5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

• 

  Affinity and Kinetic Characterization of 98096-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98096-1-RR against Human BTLA/CD272 were performed. The affinity constant is 1.84 nM.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98096-1-RR targets BTLA/CD272 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: B and T lymphocyte associated
• Calculated Molecular Weight: 289 aa, 33 kDa
• GenBank Accession Number: BC107091
• Gene Symbol: BTLA
• Gene ID (NCBI): 151888
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.