CoraLite® Plus 488-conjugated BAZ2A Recombinant antibody

BAZ2A Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230205B8

Cat no : CL488-82910-2

Synonyms

WALp3, TTF I interacting protein 5, TIP5, KIAA0314, hWALp3

---

Validation Data Gallery

• 

  IF Staining of U2OS using CL488-82910-2

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 BAZ2A antibody (CL488-82910-2, Clone: 230205B8 ) at dilution of 1:200.

• 

  FC experiment of A549 using CL488-82910-2

  1x10^6 A549 cells were intracellularly stained with 0.25 ug CoraLite® Plus 488-conjugated BAZ2A Recombinant antibody (CL488-82910-2, Clone:230205B8)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Tested Applications

• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82910-2 targets BAZ2A in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BAZ2A fusion protein Ag33963
• Full Name: bromodomain adjacent to zinc finger domain, 2A
• Calculated Molecular Weight: 211 kDa
• Observed Molecular Weight: 250-270 kDa
• GenBank Accession Number: NM_013449.4
• Gene Symbol: BAZ2A
• Gene ID (NCBI): 11176
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BAZ2A (bromodomain adjacent to zinc finger domain 2A), also known as TIP5. It is expected to be located in nucleus, and the protein is expressed at moderate levels in most tissues analyzed, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. BAZ2A is an epigenetic regulator affecting transcription of ribosomal RNA, it is also a regulatory subunit of the ATP-dependent NoRC-1 and NoRC-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed:28801535). The calculated molecular weight of BAZ2A is 210 kDa.