BAZ2A Recombinant antibody

BAZ2A Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230205B8

Cat no : 82910-2-RR

Synonyms

WALp3, TTF I interacting protein 5, TIP5, KIAA0314, hWALp3

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Validation Data Gallery

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  WB analysis using 82910-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82910-2-RR (BAZ2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 82910-2-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using BAZ2A antibody (82910-2-RR, Clone: 230205B8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of U2OS using 82910-2-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using BAZ2A antibody (82910-2-RR, Clone: 230205B8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of U-2 OS using 82910-2-RR

  (blue)1x10^6 U-2 OS were intracellularly stained with 0.25 ug Anti-Human BAZ2A (82910-2-RR, Clone:230205B8) and Alexa Fluor™ Plus 647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82910-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82910-2-RR against Human BAZ2A were performed. The affinity constant is 1.46 nM.

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, K-562 cells, MDA-MB-231 cells, PC-3 cells
• Positive IF/ICC detected in: U2OS cells, A549 cells
• Positive FC (Intra) detected in: U-2 OS

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82910-2-RR targets BAZ2A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BAZ2A fusion protein Ag33963
• Full Name: bromodomain adjacent to zinc finger domain, 2A
• Calculated Molecular Weight: 211 kDa
• Observed Molecular Weight: 250-270 kDa
• GenBank Accession Number: NM_013449.4
• Gene Symbol: BAZ2A
• Gene ID (NCBI): 11176
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAZ2A (bromodomain adjacent to zinc finger domain 2A), also known as TIP5. It is expected to be located in nucleus, and the protein is expressed at moderate levels in most tissues analyzed, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. BAZ2A is an epigenetic regulator affecting transcription of ribosomal RNA, it is also a regulatory subunit of the ATP-dependent NoRC-1 and NoRC-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed:28801535). The calculated molecular weight of BAZ2A is 210 kDa.