Autophagy Essentials Antibody Kit
Cat no : PK30004
The Autophagy Essentials Antibody Kit provides a cost-effective tool for studying key proteins involved in the autophagy pathway. Perfect for researchers starting a new project, screening multiple prospective targets or those who simply require less volume.
The Autophagy Essentials Antibody Kit contains antibodies against 5 key protein targets playing critical roles in the autophagy pathway.
|Antigen||Catalog No.||Host, clonality||Tested Reactivity||Applications||Volume|
|Beclin 1||66665-1-Ig||Mouse monoclonal||H, M, R||WB, IP, IHC, IF, ELISA||20 uL|
|LC3||81004-1-RR||Rabbit monoclonal||H, M, R, Pg||WB, IHC, IF, ELISA||20 uL|
|p62||80294-1-RR||Rabbit monoclonal||H||WB, IP, IHC, IF, ELISA||20 uL|
|ATG5||81803-1-RR||Rabbit monoclonal||H, M, R||WB, IP, IHC, ELISA||20 uL|
|LAMP1||21997-1-AP||Rabbit polyclonal||H||WB, IP, IHC, FC, ELISA||20 uL|
Also see our 'Autophagy Expanded Antibody Kit' on the following page https://www.ptglab.com/products/Autophagy-Expanded-Antibody-Kit-PK30005.htm
Store at -20°C. Stable for one year from the date of receipt.
Autophagy is a highly dynamic process consisting of the following three steps: (1) autophagosome formation, (2) autophagosome-lysosome fusion, and (3) degradation. It can be induced by multiple signaling pathways related to various triggers including nutrient deprivation, growth factor signaling, and cellular stress. The process of autophagosome formation proceeds through the steps of initiation, nucleation, elongation, closure, and ultimately fusion, each of which is regulated by various ATG proteins.
The ideal approach for measuring autophagy is to assess autophagic flux, which represents the rate of degradation of the autophagic pathway. The most widely used method for measuring autophagic flux is to detect the processing of the autophagosomal membrane protein, LC3. Analyzing autophagy substrates such as p62/SQSTM1 is often recommended in addition to measuring LC3-II turnover for accurate assessment of autophagic flux. The fusion of autophagosomes with lysosomes can be monitored by analyzing the autophagosomal marker LC3 and the lysosomal marker, LAMP simultaneously.
Click here to view our standard protocols for various applications including WB, IP, IHC, IF, FC, and ELISA.
Cited in Article as
PK30004, Autophagy Essentials Antibody Kit, Proteintech, IL, USA