CoraLite® Plus 750-conjugated Albumin Monoclonal antibody

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  WB analysis using CL750-66051

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-66051 (Albumin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using CL750-66051

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-66051 (Albumin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Albumin Monoclonal Antibody for WB

Cat No. CL750-66051

Clone No.4A1C11

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB

Synonyms

ALB, Serum albumin, HSA, 4A1C11

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Tested Applications

• Positive WB detected in: HepG2 cells, human plasma

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-66051 targets Albumin in WB applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9885

  Product name: Recombinant human Albumin protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 256-609 aa of BC034023

  Sequence: SKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL

• Full Name: albumin
• Calculated Molecular Weight: 609 aa, 69 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC034023
• Gene Symbol: Albumin
• Gene ID (NCBI): 213
• RRID: AB_3084887
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P02768
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071) In additon, glycated serum albumin is also a potential diabetes biomarker.