CoraLite®594-conjugated Albumin Monoclonal antibody

Albumin Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4A1C11

Cat no : CL594-66051

Synonyms

ALB, Serum albumin, HSA, 4A1C11

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Validation Data Gallery

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  IF Staining of mouse liver using CL594-66051

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite®594 Albumin antibody (CL594-66051, Clone: 4A1C11 ) at dilution of 1:200.

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  IF Staining of HepG2 using CL594-66051

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 Albumin antibody (CL594-66051, Clone: 4A1C11 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL594-66051

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Albumin (CL594-66051, Clone:4A1C11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF-P detected in: mouse liver tissue
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66051 targets Albumin in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Albumin fusion protein Ag9885
• Full Name: albumin
• Calculated Molecular Weight: 609 aa, 69 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC034023
• Gene Symbol: Albumin
• Gene ID (NCBI): 213
• RRID: AB_2883493
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071)