"Albumin Antibodies" Comparison
View side-by-side comparison of Albumin antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | human plasma tissue, human liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66051-1-Ig targets Albumin in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, pig, rabbit, chicken |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9885 Product name: Recombinant human Albumin protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 256-609 aa of BC034023 Sequence: SKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL Predict reactive species |
| Full Name | albumin |
| Calculated Molecular Weight | 609 aa, 69 kDa |
| Observed Molecular Weight | 66 kDa |
| GenBank Accession Number | BC034023 |
| Gene Symbol | Albumin |
| Gene ID (NCBI) | 213 |
| RRID | AB_11042320 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P02768 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071) In additon, glycated serum albumin is also a potential diabetes biomarker.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Albumin antibody 66051-1-Ig | Download protocol |
| IHC protocol for Albumin antibody 66051-1-Ig | Download protocol |
| IP protocol for Albumin antibody 66051-1-Ig | Download protocol |
| WB protocol for Albumin antibody 66051-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Total Protein antibody (Cat# 66051-1-Ig) has accumulated 68 citations across journals including Cell Metabolism, Nature Communications, Journal of Hepatology, EMBO Journal, Hepatology, and FASEB Journal. Research fields span hepatology (NAFLD, liver fibrosis, liver cancer), lipid metabolism, extracellular vesicles, ferroptosis, autophagy, diabetic retinopathy, tissue engineering, and neuroscience. Applications include WB, IF, IHC, and IP across human, mouse, rat, pig, rabbit, chicken, and sheep species.
| Species | Application | Title |
|---|---|---|
Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. | ||
Cell Metab Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans. | ||
Cell Metab AKR1D1 suppresses liver cancer progression by promoting bile acid metabolism-mediated NK cell cytotoxicity | ||
Cell Metab Hepatic FXR-FGF4 is required for bile acid homeostasis via an FGFR4-LRH-1 signal node under cholestatic stress | ||
Bioact Mater Three-dimensional bioprinted hiHeps hepatorganoids with enhanced hepatic functions for the treatment of liver failure and promotion of liver regeneration. |
Reviews
What customers say
The single review for this Albumin antibody (66051-1-Ig) is a 5-star rating from October 2019. The reviewer, Yuan Lyu, used it at a 1:5000 dilution for Western Blot on human seminal plasma and blood plasma, reporting that the antibody "is working very well" on both sample types. Overall, the feedback is positive, confirming reliable performance in WB applications with human plasma samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Yuan (Verified Customer) (10-15-2019) | This Albumin antibody is working very well on human seminal plasma and blood plasma.
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