CoraLite® Plus 488-conjugated ATPB Monoclonal antibody

ATPB Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse , Rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A11B9

Cat no : CL488-66600

Synonyms

ATP5B, ATPB, ATPMB, ATPSB, Cell and organelle markers, Mitochondria Marker, Mitochondrion Marker

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-66600

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 ATPB antibody (CL488-66600, Clone: 1A11B9 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66600 targets ATPB in IF/ICC applications and shows reactivity with Human, Mouse , Rat samples.

• Tested Reactivity: Human, Mouse , Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATPB fusion protein Ag11177
• Full Name: ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
• Calculated Molecular Weight: 529 aa, 57 kDa
• Observed Molecular Weight: 45-57 kDa
• GenBank Accession Number: BC016512
• Gene Symbol: ATPB
• Gene ID (NCBI): 506
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.