ATPB Monoclonal antibody

ATPB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse , Rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1A11B9

Cat no : 66600-1-Ig

Synonyms

ATP5B, ATPB, ATPMB, ATPSB, Cell and organelle markers, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker

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Validation Data Gallery

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  WB analysis using 66600-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66600-1-Ig (ATPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66600-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human liver using 66600-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 40x lens).

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  IHC staining of mouse skeletal muscle using 66600-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 66600-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66600-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATPB antibody (66600-1-Ig, Clone: 1A11B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 66600-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATPB antibody (66600-1-Ig, Clone: 1A11B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSCT6 cells, Raw 264.7 cells, NIH/3T3 cells
• Positive IHC detected in: human liver tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

66600-1-Ig targets ATPB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

• Tested Reactivity: Human, Mouse , Rat
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATPB fusion protein Ag11177
• Full Name: ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
• Calculated Molecular Weight: 529 aa, 57 kDa
• Observed Molecular Weight: 45-57 kDa
• GenBank Accession Number: BC016512
• Gene Symbol: ATPB
• Gene ID (NCBI): 506
• RRID: AB_2881960
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.

Publications

Species	Application	Title
rat	WB	Cell Biol Int; The effect of all-trans retinoic acid on the mitochondrial function and survival of cardiomyoblasts exposed to local photodamage; Authors - Simona Kurekova
human	WB	Mol Metab; Glucose-1,6-bisphosphate: a new gatekeeper of cerebral mitochondrial pyruvate uptake; Authors - Motahareh Solina Safari