ATPB Monoclonal antibody
ATPB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Mouse , Rat and More (2)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1A11B9
Cat no : 66600-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSCT6 cells, Raw 264.7 cells, NIH/3T3 cells |
Positive IHC detected in | human liver tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
Product Information
66600-1-Ig targets ATPB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.
Tested Reactivity | Human, Mouse , Rat |
Cited Reactivity | human, rat |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | ATPB fusion protein Ag11177 |
Full Name | ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide |
Calculated Molecular Weight | 529 aa, 57 kDa |
Observed Molecular Weight | 45-57 kDa |
GenBank Accession Number | BC016512 |
Gene Symbol | ATPB |
Gene ID (NCBI) | 506 |
RRID | AB_2881960 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ATPB antibody 66600-1-Ig | Download protocol |
IHC protocol for ATPB antibody 66600-1-Ig | Download protocol |
IF protocol for ATPB antibody 66600-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Biol Int The effect of all-trans retinoic acid on the mitochondrial function and survival of cardiomyoblasts exposed to local photodamage | ||
Mol Metab Glucose-1,6-bisphosphate: a new gatekeeper of cerebral mitochondrial pyruvate uptake |