CoraLite®594-conjugated ARL13B Polyclonal antibody

• Featured Product
• KD/KO Validated

ARL13B Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-17711

Synonyms

ARL13B, ARL2 like protein 1, ARL2L1, DKFZp686E2075, DKFZp686L2472, DKFZp686M2074, DKFZp761H079, JBTS8

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Validation Data Gallery

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  IF Staining of MDCK using CL594-17711

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CL594-17711 (ARL13B antibody) at dilution of 1:50 and CoraLite594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MDCK using CL594-17711

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CL594-17711 (ARL13B antibody) at dilution of 1:50. Cells were co-stained with CL488-66200 (acetylated Tubulin(Lys40) antibody).

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  IF Staining of hTERT-RPE1 using CL594-17711

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite®594 ARL13B antibody (CL594-17711) at dilution of 1:200, acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, green).

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Tested Applications

• Positive IF detected in: hTERT-RPE1 cells, MDCK cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-17711 targets ARL13B in IF applications and shows reactivity with human, mouse, rat, Canine samples.

• Tested Reactivity: human, mouse, rat, Canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARL13B fusion protein Ag12015
• Full Name: ADP-ribosylation factor-like 13B
• Calculated Molecular Weight: 48 kDa
• GenBank Accession Number: BC094725
• Gene Symbol: ARL13B
• Gene ID (NCBI): 200894
• RRID: AB_2883407
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). CoraLite594-Conjugated ARL13B antibody can be used to mark the cilia(red). Ex: 593nm, Em: 614nm