CoraLite® Plus 488-conjugated ARL13B Polyclonal antibody

• Featured Product
• KD/KO Validated

ARL13B Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

2

Cat no : CL488-17711

Synonyms

ARL13B, ARL2 like protein 1, ARL2L1, ARL2-like protein 1, ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1

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Validation Data Gallery

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  IF Staining of hTERT-RPE1 using CL488-17711

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite® Plus 488 ARL13B antibody (CL488-17711) at dilution of 1:200, CEP250/CNAP1 antibody (14498-1-AP, orange), SCLT1 antibody (14875-1-AP, Magenta).

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  IF Staining of MDCK using CL488-17711

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CL488-17711 (ARL13B antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of hTERT-RPE1 using CL488-17711

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 ARL13B antibody (CL488-17711) at dilution of 1:200, CoraLite®594 Acetyl-Tubulin (Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

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Tested Applications

• Positive IF/ICC detected in: hTERT-RPE1 cells, MDCK cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

CL488-17711 targets ARL13B in IF/ICC applications and shows reactivity with human, mouse, rat, Canine samples.

• Tested Reactivity: human, mouse, rat, Canine
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARL13B fusion protein Ag12015
• Full Name: ADP-ribosylation factor-like 13B
• Calculated Molecular Weight: 48 kDa
• GenBank Accession Number: BC094725
• Gene Symbol: ARL13B
• Gene ID (NCBI): 200894
• RRID: AB_2883085
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). CoraLite488-Conjugated ARL13B antibody can be used to mark the cilia(green). Ex: 488nm, Em: 515nm

Publications

Species	Application	Title
human	IF	Mol Oncol; Interferon regulatory factor 4 modulates epigenetic silencing and cancer-critical pathways in melanoma cells; Authors - Ulduz Sobhiafshar
mouse	IF	Neurosci Res; Phenotypic characterization of Cdkl5-knockdown neurons establishes elongated cilia as a functional assay for CDKL5 Deficiency Disorder.; Authors - Alessia Di Nardo