ALDH3A1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ALDH3A1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D4G7

Cat no : 68036-1-PBS

Synonyms

Aldehyde dehydrogenase 3, ALDH3, ALDH3A1, ALDHIII

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Validation Data Gallery

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  WB analysis of A549 using 68036-1-Ig (same clone as 68036-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 68036-1-Ig (ALDH3A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68036-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 68036-1-Ig (same clone as 68036-1-PBS)

  WB result of ALDH3A1 antibody (68036-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH3A1 transfected A549 cells. This data was developed using the same antibody clone with 68036-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 68036-1-Ig (same clone as 68036-1-PBS)

  WB result of ALDH3A1 antibody (68036-1-Ig; 1:80000; room temperature for 1.5 hours) with wild-type and ALDH3A1 knockout A549 cells. This data was developed using the same antibody clone with 68036-1-PBS in a different storage buffer formulation.

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  WB analysis of human saliva using 68036-1-Ig (same clone as 68036-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 68036-1-Ig (ALDH3A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68036-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 68036-1-Ig (same clone as 68036-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68036-1-Ig (ALDH3A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68036-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 68036-1-Ig (same clone as 68036-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68036-1-Ig (ALDH3A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68036-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 68036-1-Ig (same clone as 68036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68036-1-Ig (ALDH3A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68036-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 68036-1-Ig (same clone as 68036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68036-1-Ig (ALDH3A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68036-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 68036-1-Ig (same clone as 68036-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ALDH3A1 antibody (68036-1-Ig, Clone: 1D4G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68036-1-PBS in a different storage buffer formulation.

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Product Information

68036-1-PBS targets ALDH3A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ALDH3A1 fusion protein Ag8125
• Full Name: aldehyde dehydrogenase 3 family, memberA1
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC004370
• Gene Symbol: ALDH3A1
• Gene ID (NCBI): 218
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

ALDH3A1(Aldehyde dehydrogenase, dimeric NADP-preferring), also named as ALDH3, is a member of the ALDH superfamily, which consists of NAD(P)+-dependent enzymes that catalyse the oxidation of a wide variety of endogenously produced and exogenous aldehydes to their corresponding acids. It is expressed at high concentrations in the mammalian cornea and it is believed that it protects this vital tissue and the rest of the eye against UVlight-induced damage.(PMID:12943535). It can exsit as a dimer(PMID:16919242).