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ALDH3A1 Monoclonal antibody

ALDH3A1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D4G7

Cat no : 68036-1-Ig

Synonyms

Aldehyde dehydrogenase 3, ALDH3, ALDH3A1, ALDHIII

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using ALDH3A1 Monoclonal antibody (68036-1-Ig)

    WB analysis of A549 using 68036-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68036-1-Ig (ALDH3A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using ALDH3A1 Monoclonal antibody (68036-1-Ig)

    WB analysis of A549 using 68036-1-Ig

    WB result of ALDH3A1 antibody (68036-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH3A1 transfected A549 cells.

  • Western Blot (WB) analysis of A549 cells using ALDH3A1 Monoclonal antibody (68036-1-Ig)

    WB analysis of A549 using 68036-1-Ig

    WB result of ALDH3A1 antibody (68036-1-Ig; 1:80000; room temperature for 1.5 hours) with wild-type and ALDH3A1 knockout A549 cells.

  • Western Blot (WB) analysis of LNCaP cells using ALDH3A1 Monoclonal antibody (68036-1-Ig)

    WB analysis of LNCaP using 68036-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68036-1-Ig (ALDH3A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ALDH3A1 Monoclonal antibody (68036-1-Ig)

    WB analysis of HeLa using 68036-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68036-1-Ig (ALDH3A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human saliva using ALDH3A1 Monoclonal antibody (68036-1-Ig)

    WB analysis of human saliva using 68036-1-Ig

    human saliva were subjected to SDS PAGE followed by western blot with 68036-1-Ig (ALDH3A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ALDH3A1 Monoclonal antibody (68036-1-Ig)

    IHC staining of human kidney using 68036-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68036-1-Ig (ALDH3A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ALDH3A1 Monoclonal antibody (68036-1-Ig)

    IHC staining of human kidney using 68036-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68036-1-Ig (ALDH3A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ALDH3A1 Monoclonal antibody (68036-1-Ig)

    IF Staining of HEK-293 using 68036-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ALDH3A1 antibody (68036-1-Ig, Clone: 1D4G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, LNCaP cells, HeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68036-1-Ig targets ALDH3A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ALDH3A1 fusion protein Ag8125
Full Name aldehyde dehydrogenase 3 family, memberA1
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC004370
Gene Symbol ALDH3A1
Gene ID (NCBI) 218
RRIDAB_2918778
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDH3A1(Aldehyde dehydrogenase, dimeric NADP-preferring), also named as ALDH3, is a member of the ALDH superfamily, which consists of NAD(P)+-dependent enzymes that catalyse the oxidation of a wide variety of endogenously produced and exogenous aldehydes to their corresponding acids. It is expressed at high concentrations in the mammalian cornea and it is believed that it protects this vital tissue and the rest of the eye against UVlight-induced damage.(PMID:12943535). It can exsit as a dimer(PMID:16919242).

Protocols

Product Specific Protocols
WB protocol for ALDH3A1 antibody 68036-1-IgDownload protocol
IHC protocol for ALDH3A1 antibody 68036-1-IgDownload protocol
IF protocol for ALDH3A1 antibody 68036-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols