- Featured Product
- KD/KO Validated
ALDH3A1 Monoclonal antibody
ALDH3A1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1D4G7
Cat no : 68036-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, LNCaP cells, HeLa cells |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68036-1-Ig targets ALDH3A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | ALDH3A1 fusion protein Ag8125 |
Full Name | aldehyde dehydrogenase 3 family, memberA1 |
Calculated Molecular Weight | 50 kDa |
Observed Molecular Weight | 50 kDa |
GenBank Accession Number | BC004370 |
Gene Symbol | ALDH3A1 |
Gene ID (NCBI) | 218 |
RRID | AB_2918778 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ALDH3A1(Aldehyde dehydrogenase, dimeric NADP-preferring), also named as ALDH3, is a member of the ALDH superfamily, which consists of NAD(P)+-dependent enzymes that catalyse the oxidation of a wide variety of endogenously produced and exogenous aldehydes to their corresponding acids. It is expressed at high concentrations in the mammalian cornea and it is believed that it protects this vital tissue and the rest of the eye against UVlight-induced damage.(PMID:12943535). It can exsit as a dimer(PMID:16919242).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ALDH3A1 antibody 68036-1-Ig | Download protocol |
IHC protocol for ALDH3A1 antibody 68036-1-Ig | Download protocol |
IF protocol for ALDH3A1 antibody 68036-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |