CoraLite®594-conjugated ACTN4 Monoclonal antibody

• Featured Product
• KD/KO Validated

ACTN4 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E12B8

Cat no : CL594-66628

Synonyms

ACTININ 4, actinin, alpha 4, ACTN4, Alpha actinin 4, DKFZp686K23158, F actin cross linking protein, FSGS, FSGS1, Non muscle alpha actinin 4

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Validation Data Gallery

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  FC experiment of HeLa using CL594-66628

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ACTN4 (CL594-66628, Clone:1E12B8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66628 targets ACTN4 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACTN4 fusion protein Ag13549
• Full Name: actinin, alpha 4
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 105 and 80 kDa
• GenBank Accession Number: BC005033
• Gene Symbol: ACTN4
• Gene ID (NCBI): 81
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 105 and 80 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177)