ACTN4 Monoclonal antibody

• Featured Product
• KD/KO Validated

ACTN4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1E12B8

Cat no : 66628-1-Ig

Synonyms

ACTININ 4, actinin, alpha 4, ACTN4, Alpha actinin 4, F actin cross linking protein, FSGS, FSGS1, Non muscle alpha actinin 4

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Validation Data Gallery

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  WB analysis using 66628-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66628-1-Ig (ACTN4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66628-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66628-1-Ig (ACTN4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66628-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66628-1-Ig (ACTN4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66628-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66628-1-Ig (ACTN4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66628-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66628-1-Ig (ACTN4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66628-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66628-1-Ig (ACTN4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, LNCaP cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, K-562 cells, pig brain tissue, mouse brain tissue, rat brain tissue, chicken brain tissue
• Positive IHC detected in: human breast cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

66628-1-Ig targets ACTN4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACTN4 fusion protein Ag13549
• Full Name: actinin, alpha 4
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 105 and 80 kDa
• GenBank Accession Number: BC005033
• Gene Symbol: ACTN4
• Gene ID (NCBI): 81
• RRID: AB_2881988
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 105 and 80 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177)

Publications

Species	Application	Title
human	IF	Int Immunopharmacol; 1β-Hydroxyalantolactone from Inulae Flos alleviated the progression of pulmonary fibrosis via inhibiting JNK/FOXO1/NF-κB pathway; Authors - Bing Yu
human	WB	Environ Toxicol; TRIP13 exerts a cancer-promoting role in cervical cancer by enhancing Wnt/β-catenin signaling via ACTN4; Authors - Xiaoying Liu
	WB,IF	J Physiol Biochem; Dysregulated expression of ACTN4 contributes to endothelial cell injury via the activation of the p38-MAPK/p53 apoptosis pathway in preeclampsia.; Authors - Jianlin Zhao ; KD Validated
human	WB,IF,IP	Front Oncol; The deubiquitinating enzyme MINDY2 promotes pancreatic cancer proliferation and metastasis by stabilizing ACTN4 expression and activating the PI3K/AKT/mTOR signaling pathway; Authors - Peng Liu