ACSF2 Recombinant antibody, PBS Only (Detector)

ACSF2 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241208D9

Cat no : 84033-3-PBS

Synonyms

UNQ493/PRO1009, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, EC:6.2.1.2, ACSF 2, 241208D9

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Validation Data Gallery

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  WB analysis using 84033-3-RR (same clone as 84033-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84033-3-RR (ACSF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84033-3-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 84033-3-RR (same clone as 84033-3-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug ACSF2 Recombinant antibody (84033-3-RR, Clone:241208D9) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84033-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00952-1

  Cytometric bead array standard curve of MP00952-1, ACSF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84033-2-PBS. Detection antibody: 84033-3-PBS. Standard: Ag9199. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP00952-3

  Cytometric bead array standard curve of MP00952-3, ACSF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84033-1-PBS. Detection antibody: 84033-3-PBS. Standard: Ag9199. Range: 2.5-80 ng/mL.

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Product Information

84033-3-PBS targets ACSF2 as part of a matched antibody pair:

MP00952-1: 84033-2-PBS capture and 84033-3-PBS detection (validated in Cytometric bead array)

MP00952-3: 84033-1-PBS capture and 84033-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ACSF2 fusion protein Ag9199
• Full Name: acyl-CoA synthetase family member 2
• Calculated Molecular Weight: 615 aa, 68 kDa
• Observed Molecular Weight: 51-71 kDa
• GenBank Accession Number: BC012053
• Gene Symbol: ACSF2
• Gene ID (NCBI): 80221
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ACSF2 (encoded by acyl-CoA synthetase family member 2) belongs to the acyl-CoA synthetase (ACS) family, activating fatty acids by forming a thioester bond with CoA.