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ACSF2 Recombinant antibody

ACSF2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241208D9

Cat no : 84033-3-RR

Synonyms

UNQ493/PRO1009, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, EC:6.2.1.2, ACSF 2, 241208D9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACSF2 Recombinant antibody (84033-3-RR)

    WB analysis using 84033-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84033-3-RR (ACSF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using ACSF2 Recombinant antibody (84033-3-RR)

    FC experiment of A549 using 84033-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug ACSF2 Recombinant antibody (84033-3-RR, Clone:241208D9) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inJurkat cells, SGC-7901 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84033-3-RR is ACSF2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ACSF2 fusion protein Ag9199
Full Name acyl-CoA synthetase family member 2
Calculated Molecular Weight 615 aa, 68 kDa
Observed Molecular Weight51-71 kDa
GenBank Accession NumberBC012053
Gene Symbol ACSF2
Gene ID (NCBI) 80221
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACSF2 (encoded by acyl-CoA synthetase family member 2) belongs to the acyl-CoA synthetase (ACS) family, activating fatty acids by forming a thioester bond with CoA.

Protocols

Product Specific Protocols
WB protocol for ACSF2 antibody 84033-3-RRDownload protocol
FC protocol for ACSF2 antibody 84033-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols