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Multi-rAb CoraLite® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L)

Multi-rAb Goat Anti-rabbit recombinant secondary antibody is a mixture of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on rabbit IgG heavy and light chain.

Applications

IF, FC

Conjugate

CoraLite® Plus 647

Host

Goat

Reactivity

Rabbit

Cat no : RGAR005

Validation Data Gallery

  • Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-GM130 polyclonal antibody (11308-1-AP, 1:200) Multi-rAb CoraLite® Plus 647 conjugated Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005,1:800) was used for detection. Nucleus was stained with DAPI (blue).    The experiment was performed in Chromotek’s lab and image was recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-GM130 polyclonal antibody (11308-1-AP, 1:200) Multi-rAb CoraLite® Plus 647 conjugated Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005,1:800) was used for detection. Nucleus was stained with DAPI (blue).    The experiment was performed in Chromotek’s lab and image was recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse small intestine tissue using smooth muscle actin antibody (14395-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005).

    Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse small intestine tissue using smooth muscle actin antibody (14395-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005).

  • Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005).

    Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005).

  • Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, magenta) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, green). Multi-rAb CoraLite® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005, 1:500) and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002, 1:500) were used for detection.

    Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, magenta) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, green). Multi-rAb CoraLite® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005, 1:500) and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002, 1:500) were used for detection.

  • Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, magenta) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, green). Multi-rAb CoraLite® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005, 1:500) and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002, 1:500) were used for detection.

    Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, magenta) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, green). Multi-rAb CoraLite® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005, 1:500) and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002, 1:500) were used for detection.

  • Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (26975-1-AP, 1:200, magenta) and mouse anti-Calbindin-D28k monoclonal antibody (66394-1-Ig, 1:200, green). Multi-rAb CoraLite® Plus 647 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR005, 1:500) and Multi-rAb CoraLite® Plus 488 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM002, 1:500) .

    Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (26975-1-AP, 1:200, magenta) and mouse anti-Calbindin-D28k monoclonal antibody (66394-1-Ig, 1:200, green). Multi-rAb CoraLite® Plus 647 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR005, 1:500) and Multi-rAb CoraLite® Plus 488 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM002, 1:500) .

  • Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, magenta) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, green). Multi-rAb CoraLite® Plus 647 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR005, 1:500) and Multi-rAb CoraLite® Plus 488 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM002, 1:500) .

    Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, magenta) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, green). Multi-rAb CoraLite® Plus 647 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR005, 1:500) and Multi-rAb CoraLite® Plus 488 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM002, 1:500) .

  • 1X10^6 MOLT4 were surface stained with 0.2 ug Anti-HLA class I rabbit polyclonal antibody (15240-1-AP) and Rabbit IgG Isotype Control 30000-0-AP. Multi-rAb CoraLite® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) RGAR005 was used at 1:500 for detection.

    1X10^6 MOLT4 were surface stained with 0.2 ug Anti-HLA class I rabbit polyclonal antibody (15240-1-AP) and Rabbit IgG Isotype Control 30000-0-AP. Multi-rAb CoraLite® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) RGAR005 was used at 1:500 for detection.

Product Information

RGAR005 targets Rabbit IgG (H+L) in FC and IF. It is higly specific to rabbit IgG and shows minimal reactivity for mouse, rat, human, pig and bovine IgGs.

Concentration 0.5 mg/mL
Applications IF, FC
Host Species Goat
Species Reactivity Rabbit
Physical State Liquid
Suggested Dilution Range 1:200-1:1000 for IF and FC
Purity The antibody was purified from culture media supernatant by immunoaffinity chromatography using Protein G beads.
Storage Buffer PBS with 50% glycerol, 10 mg/mL BSA, 0.1% Proclin-300, pH 7.4.
Storage Instructions Store at -20°C. Stable for one year after shipment.
FluorophoreCoraLite® Plus 647, Amax=654 nm, Emax=674 nm
ClonalityMulticlonal recombinant
RRID AB_3073509
NoteThis product is for research use only,not for diagnostic or therapeutic use.

Background

Multi-rAb Recombinant Secondary Antibodies are mixtures of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on the same IgG. Each recombinant clone in the final multiclonal mixture is carefully selected after rigorous characterization and screening to ensure the highest level of performance. ​The selected clones are mixed in various combinations, each of which is then functionally characterized in multiple applications to select the final multiclonal mixture with optimal performance. Each lot of Multi-rAb Secondary Antibodies is produced by mixing the same high performing recombinant clones every time to ensure that they have high batch-to-batch consistency, which in turn results in high reproducibility of experimental data.

Publications

Title

ACS Appl Mater Interfaces

Self-Delivery Nanobooster to Enhance Immunogenic Cell Death for Cancer Chemoimmunotherapy

Authors - Chunmei Yan
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Angel (Verified Customer) (08-30-2024)

Didn't quite work for us, but we may need to adjust some aspects of our protocol.

  • Applications: Immunofluorescence
  • Cell Tissue Type: breast epithelial, tumorigenic
FH

Paula (Verified Customer) (07-30-2024)

Used this secondary antibody (1:250) for ICC on immortalised cell line (mouse).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: Mouse (immortalised cell line)
FH

Adam (Verified Customer) (04-19-2024)

For the price this product was a great alternative to AF488. The quality of the images obtained from using this 488 secondary antibody was good.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: Molm-13 AML cell line