ACSF2 Recombinant antibody

ACSF2 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241208D9

Cat no : 84033-3-RR

Synonyms

UNQ493/PRO1009, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, EC:6.2.1.2, ACSF 2, 241208D9

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Validation Data Gallery

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  WB analysis using 84033-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84033-3-RR (ACSF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of A549 using 84033-3-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug ACSF2 Recombinant antibody (84033-3-RR, Clone:241208D9) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Jurkat cells, SGC-7901 cells
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84033-3-RR is ACSF2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ACSF2 fusion protein Ag9199
• Full Name: acyl-CoA synthetase family member 2
• Calculated Molecular Weight: 615 aa, 68 kDa
• Observed Molecular Weight: 51-71 kDa
• GenBank Accession Number: BC012053
• Gene Symbol: ACSF2
• Gene ID (NCBI): 80221
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACSF2 (encoded by acyl-CoA synthetase family member 2) belongs to the acyl-CoA synthetase (ACS) family, activating fatty acids by forming a thioester bond with CoA.