CoraLite® Plus 647-conjugated ACAT2 Polyclonal antibody

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  FC experiment of HepG2 using CL647-14755

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human ACAT2 (CL647-14755) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

ACAT2 Polyclonal Antibody for FC (Intra)

Cat No. CL647-14755

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

EC:2.3.1.9, Cytosolic acetoacetyl-CoA thiolase, ACTL, Acetyl-CoA transferase-like protein, Acetyl-CoA acetyltransferase, cytosolic

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Tested Applications

• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-14755 targets ACAT2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACAT2 fusion protein Ag6503
• Full Name: acetyl-Coenzyme A acetyltransferase 2
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 40-42 kDa
• GenBank Accession Number: BC000408
• Gene Symbol: ACAT2
• Gene ID (NCBI): 39
• RRID: AB_2934902
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BWD1
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Acyl-CoA: cholesterolacyltransferase (ACAT) is an in tracellular membrane-bound enzyme that uses cholesterol and long chain fatty acyl-coenzyme A (CoA) as substrates to produce cholesteryl esters. Two isoforms of the enzyme ACAT, i.e., ACAT1 and ACAT2, are known to exist. ACAT2, which is expressed in liver and intestine cells, has been found to play an important role in intracellular cholesterol homeostasis and univariate survival analyses have shown that ACAT2 expression is associated with a poor clear cell renal cell carcinoma associated survival.