Validation Data Gallery
|Positive WB detected in||Caco-2 cells, HepG2 cells, K-562 cells, MOLT-4 cells, mouse liver, rat liver|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human liver tissue, human lung tissue, human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14755-1-AP targets ACAT2 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACAT2 fusion protein Ag6503|
|Full Name||acetyl-Coenzyme A acetyltransferase 2|
|Calculated molecular weight||41 kDa|
|Observed molecular weight||40-42 kDa|
|GenBank accession number||BC000408|
|Gene ID (NCBI)||39|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol.
Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.
Cancer Cell Int
Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer
Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice.
J Agric Food Chem
Oral Administration of Succinoglycan Riclin Improves Diet-Induced Hypercholesterolemia in Mice.
Food Res Int
Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sarah-Eve (Verified Customer) (03-01-2023)
Very good working on PVDF membrane, blocking solution milk 5%