ACAT2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ACAT2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 14755-1-AP

Synonyms

ACAT2, ACTL

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Validation Data Gallery

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  WB analysis of HepG2 using 14755-1-AP

  WB result of ACAT2 antibody (14755-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT2 transfected HepG2 cells.

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  WB analysis using 14755-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14755-1-AP (ACAT2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14755-1-AP

  IP Result of anti-ACAT2 (IP:14755-1-AP, 4ug; Detection:14755-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human liver using 14755-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 14755-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 14755-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 14755-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 14755-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 14755-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 40x lens).

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  FC experiment of HepG2 using 14755-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug ACAT2 antibody (14755-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: Caco-2 cells, HepG2 cells, K-562 cells, MOLT-4 cells, mouse liver, rat liver
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver tissue, human lung tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 6 publications below
• IHC: See 2 publications below

Product Information

14755-1-AP targets ACAT2 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACAT2 fusion protein Ag6503
• Full Name: acetyl-Coenzyme A acetyltransferase 2
• Calculated molecular weight: 41 kDa
• Observed molecular weight: 40-42 kDa
• GenBank accession number: BC000408
• Gene symbol: ACAT2
• Gene ID (NCBI): 39
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		Cell Res; Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol.; Authors - Yanjing Guo
human	WB	Cell Res; Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.; Authors - De Huang
human	IHC	Cancer Cell Int; Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer; Authors - Enkui Zhang
mouse	WB	iScience; Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice.; Authors - George D Dalton
mouse	WB	J Agric Food Chem; Oral Administration of Succinoglycan Riclin Improves Diet-Induced Hypercholesterolemia in Mice.; Authors - Yunxia Yang
human	WB	Food Res Int; Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo.; Authors - Suwen Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sarah-Eve (Verified Customer) (03-01-2023)

Very good working on PVDF membrane, blocking solution milk 5%

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human pulmonary artery smooth muscle cells

ACAT2 Antibody Western Blot validation (1:2000 dilution) in Human pulmonary artery smooth muscle cells (Cat no:14755-1-AP)