Biotin-conjugated 6*His, His-Tag Monoclonal antibody

6*His, His-Tag Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

ELISA

Conjugate

Biotin

CloneNo.

1B7G5

Cat no : Biotin-66005

Synonyms

6*His, His-Tag

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Validation Data Gallery

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  ELISA experiment of Recombinant protein using Biotin-66005

  Biotin-66005 was tested by ELISA. Ag19172(GST-3*MYC-6*HIS-3*FLAG-6*HIS-3*HA-6*HIS)was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of Biotin-66005 (start dilution 1:1000. Bound antibodies were detected with Streptavidin Poly-HRP(1:5000)followed by incubation with HRP Substrate, terminated with 2M H2SO4, then measuring the resulting absorbance at 450 nm.

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Tested Applications

• Positive ELISA detected in: Recombinant protein

Recommended dilution

• Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-66005 targets 6*His, His-Tag in ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: 6*His, His-Tag
• Calculated Molecular Weight: 1 kDa
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.