6*His, His-Tag Antibody 64 Publications

Mouse Monoclonal| Catalog number: 66005-1-Ig |CloneNo.: 1B7G5

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Freight/Packing

Size: 09µ/gram

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Species specificity:
recombinant protein

Positive WB detected in:
recombinant protein

Positive IP detected in:
Transfected HEK-293 cells

Positive IF detected in:
Transfected HEK-293 cells

Recommended dilution:
WB : 1:5000-1:50000
IP : 1:5000-1:50000
IF : 1:10-1:100

Product Information


Source:
Mouse

Purification method:
Protein G purification

Isotype:
IgG1

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Peptide

Full name:
6*His, His-Tag

Calculated molecular weight:
1 kDa

Observed molecular weight:

GenBank accession number:

Gene ID (NCBI):

Gene symbol

Synonyms
6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag, HISTIDINE TAGGED
Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.


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