HRP-conjugated 6*His, His-Tag Monoclonal antibody
6*His, His-Tag Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG1
Reactivity
recombinant protein
Applications
WB, IP, CoIP, ELISA, Blocking assay
Conjugate
HRP
153
CloneNo.
1B7G5
Cat no : HRP-66005
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Recombinant protein protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 88 publications below |
| IP | See 2 publications below |
| ELISA | See 5 publications below |
| CoIP | See 4 publications below |
Product Information
HRP-66005 targets 6*His, His-Tag in WB, IP, CoIP, ELISA, Blocking assay applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | 6xHis Peptide |
| Full Name | 6*His, His-Tag |
| Calculated Molecular Weight | 1 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2857904 |
| Conjugate | HRP |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. This His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells. This antibody is conjugated with HRP.
Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP 6*His, His-Tag antibody HRP-66005 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Immunity Detection of SARS-CoV-2-Specific Humoral and Cellular Immunity in COVID-19 Convalescent Individuals. | ||
Immunity Decreased Expression of the Host Long-Noncoding RNA-GM Facilitates Viral Escape by Inhibiting the Kinase activity TBK1 via S-glutathionylation. | ||
Nat Commun Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence | ||
Nat Commun Antioxidant hepatic lipid metabolism can be promoted by orally administered inorganic nanoparticles | ||
Nat Commun The structure of a Plasmodium vivax Tryptophan Rich Antigen domain suggests a lipid binding function for a pan-Plasmodium multi-gene family |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Zaneta (Verified Customer) (03-06-2023) | I used the antibody to detect small amount of 6xhis and 8xhis - tagged proteins from HEK cells and SF9 cells, on WesternBlot. It works perfect, fast and efficient!
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FH Tom (Verified Customer) (02-03-2023) | Works great to detect recombinant protein from bacteria
|
FH Javier (Verified Customer) (12-16-2022) | Even though there was an issue with the shipment and when I received the antibody it was at room temperature, it has worked perfectly fine. Because of the delay I have used it at a 1:5000 dilution an incubated it for 1h 30 min at room temperature. I would totally recommend it.
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FH Tom (Verified Customer) (07-12-2021) | Blocked membrane overnight in 5% BSA in TBS. Tried at both 1:1000 and 1:10,000 dilutions in 5% BSA in TBST for 2.5h at room temperature and got great, specific bands for both dilutions.
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FH Apoorv (Verified Customer) (12-15-2019) | Used to detect protein in Western blots
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FH Soo (Verified Customer) (10-24-2019) | we use this antibody to detect recombinant proteins which has his tag. Used it for western and ELISA and Flow , works well for us. It is cheaper than other brand's his tag antibody, another reason we like this product.
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FH Angel (Verified Customer) (09-09-2019) | It is the best antibody I've ever used for quick check of protein expression and size for heterologous protein production and purification.
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FH Ryan (Verified Customer) (02-04-2019) | This His-tag-HRP conjugated antibody is the single best His-tag antibody I have ever used. I have used dilutions from 1:1000 to 1:25,000 with fantastic results. The background with this antibody is minimal and the sensitivity is superb. This image shows four different proteins with both 6xHis and 8xHis-tags. I have used the antibody in milk and 5% BSA. Incubation times have been as short as 1 hour and as long as 24 hours; the results are always the same. Being conjugated, it can cut the time of a typical Western blot down by a few hours. My standard procedure with this antibody is 1:8,000 in 5% BSA for 1 hour with three 10 minute washes and a 5 second exposure using Cell Signaling SignalFire ECL.
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FH Jennifer (Verified Customer) (12-08-2018) | Excellent product. We achieved to see the production of our protein of interest in an accurate way. Some previous secondary antibodies gave us a lot of background; this one works better.
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