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FITC Plus Anti-Human CD107a / LAMP1 (H4A3)

CD107a / LAMP1 Monoclonal Antibody for FC, IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC, IF/ICC, FC (Intra)

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

H4A3

Cat no : FITC-65051

Synonyms

CD107a, LAMP1, LAMP-1, LAMP 1, H4A3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FITC Plus Anti-Human CD107a / LAMP1 (H4A3) (FITC-65051)

    IF Staining of HeLa using FITC-65051

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FITC-65051 (CD107a antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using FITC Plus Anti-Human CD107a / LAMP1 (H4A3) (FITC-65051)

    FC experiment of HeLa using FITC-65051

    1x10^6 HeLa cells were intracellularly stained with 10 ul FITC Plus Anti-Human CD107a / LAMP1 (H4A3) (FITC-65051, Clone:H4A3)(red), or 10 ul FITC Plus Mouse IgG1 Isotype Control (MOPC-21) (FITC-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD107a / LAMP1 (H4A3) (FITC-65051)

    FC experiment of human PBMCs using FITC-65051

    1X10^6 PHA-activated human PBMCs were surface stained with 5 ul FITC Plus Plus Anti-Human CD107a (FITC-65051, Clone: H4A3). Unactivated PBMCs were used as negative control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD107a / LAMP1 (H4A3) (FITC-65051)

    FC experiment of human PBMCs using FITC-65051

    1X10^6 PHA-activated human PBMCs were surface stained with 5 ul FITC Plus Plus Anti-Human CD107a (FITC-65051, Clone: H4A3). Unactivated PBMCs were used as negative control. Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) :
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65051 targets CD107a / LAMP1 in FC, IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human adherent peripheral blood cells
Full Name lysosomal-associated membrane protein 1
GenBank Accession NumberBC006345
Gene Symbol LAMP1
Gene ID (NCBI) 3916
RRIDAB_2883741
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

Protocols

Product Specific Protocols
IF protocol for FITC Plus CD107a / LAMP1 antibody FITC-65051Download protocol
Standard Protocols
Click here to view our Standard Protocols