CoraLite® Plus 405 Anti-Human CD107a / LAMP1 (H4A3)

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  FC experiment of HeLa using CL405-65051

  1X10^6 HeLa cells were intracellularly stained with 5 ul CoraLite® Plus 405 Anti-Human CD107a / LAMP1 (CL405-65051, Clone:H4A3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• 

  FC experiment of HeLa using CL405-65051

  1X10^6 HeLa cells were intracellularly stained with 5 ul CoraLite® Plus 405 Anti-Human CD107a / LAMP1 (CL405-65051, Clone:H4A3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• 

  FC experiment of Jurkat using CL405-65051

  1x10^6 Jurkat cells were intracellularly stained with 5 ul CoraLite® Plus 405 Anti-Human CD107a / LAMP1 (H4A3) (CL405-65051, Clone: H4A3) (red) or CoraLite® Plus 405 Mouse IgG1 Isotype Control (MOPC-21) (CL405-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

CD107a / LAMP1 Monoclonal Antibody for FC (Intra)

Cat No. CL405-65051

Clone No.H4A3

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC (Intra)

Synonyms

CD107a, LAMP1, CD107 antigen-like family member A, CD107a/LAMP1, H4A3

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells, Jurkat cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL405-65051 targets CD107a / LAMP1 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Human adherent peripheral blood cells

• Full Name: lysosomal-associated membrane protein 1
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: BC006345
• Gene Symbol: LAMP1
• Gene ID (NCBI): 3916
• ENSEMBL Gene ID: ENSG00000185896
• RRID: AB_2934345
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P11279
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).