Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | Jurkat cells |
| Positive FC detected in | Thrombin treated human peripheral blood platelets |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65596-1-MR targets CD107a / LAMP1 in IF/ICC, FC, FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Human adherent peripheral blood cells Predict reactive species |
| Full Name | lysosomal-associated membrane protein 1 |
| Calculated Molecular Weight | 45 kDa |
| GenBank Accession Number | BC006345 |
| Gene Symbol | LAMP1 |
| Gene ID (NCBI) | 3916 |
| ENSEMBL Gene ID | ENSG00000185896 |
| RRID | AB_3670344 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P11279 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD107a / LAMP1 antibody 65596-1-MR | Download protocol |
| IF protocol for CD107a / LAMP1 antibody 65596-1-MR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-Human CD107a/LAMP1 (H4A3) Mouse IgG2a Recombinant Antibody (Cat# 65596-1-MR) has 3 citations published in Advanced Science, International Immunopharmacology, and Cell Reports Medicine. The research fields span NK cell glycoengineering for cancer immunotherapy, immune evasion mechanisms in clear cell renal cell carcinoma via Siglec-7 checkpoint engagement on CD8⁺ T cells, and c-Met targeting aptamer-drug conjugates for anti-tumor efficacy. All cited applications were flow cytometry (FC).
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) One-Step Glycoengineering of NK Cells With High-Affinity Siglec Ligands for Cancer Immunotherapy | ||
Int Immunopharmacol Polysialylated CD56 drives immune evasion in clear cell renal cell carcinoma via engagement of the Siglec-7 checkpoint on CD8(+) T cells. | ||
Cell Rep Med Fast tumor accumulation and payload release of c-Met targeting aptamer-drug conjugate enables robust anti-tumor efficacy. |
