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CoraLite® Plus 750 Anti-Human CD45 Rabbit Recombinant Antibody

CD45 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

241670E10

Cat no : CL750-98117

Synonyms

L CA, GP180, EC:3.1.3.48, CD45R, B220

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using CoraLite® Plus 750 Anti-Human CD45 Rabbit Recombin (CL750-98117)

    FC experiment of human peripheral blood leukocytes using CL750-98117

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD45 Rabbit Recombinant Antibody (CL750-98117, Clone: 241670E10) or CoraLite® Plus 750 Rabbit IgG Isotype Control Recombinant Antibody (CL750-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using CoraLite® Plus 750 Anti-Human CD45 Rabbit Recombin (CL750-98117)

    FC experiment of human peripheral blood leukocytes using CL750-98117

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD45 Rabbit Recombinant Antibody (CL750-98117, Clone: 241670E10) red) or CoraLite® Plus 750 Rabbit IgG Isotype Control Recombinant Antibody (CL750-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Tested Applications

Positive FC detected inFC receptor blocked human peripheral blood leukocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL750-98117 is CD45 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name protein tyrosine phosphatase, receptor type, C
Gene Symbol CD45
Gene ID (NCBI) 5788
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Protocols

Product Specific Protocols
FC protocol for CL Plus 750 CD45 antibody CL750-98117Download protocol
Standard Protocols
Click here to view our Standard Protocols