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CoraLite®594-conjugated CD23 Monoclonal antibody

CD23 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4C7C9

Cat no : CL594-60208

Synonyms

BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated CD23 Monoclonal antibody (CL594-60208)

    IF Staining of human tonsillitis using CL594-60208

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-60208 (CD23 antibody) at dilution of 1:50 .

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated CD23 Monoclonal antibody (CL594-60208)

    IF Staining of human tonsillitis using CL594-60208

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-60208 (CD23 antibody) at dilution of 1:50 .

  • Immunofluorescence (IF) / fluorescent staining of human lymphoma tissue using CoraLite®594-conjugated CD23 Monoclonal antibody (CL594-60208)

    IF Staining of human lymphoma using CL594-60208

    Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using CoraLite®594 CD23 antibody (CL594-60208, Clone: 4C7C9 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human lymphoma tissue using CoraLite®594-conjugated CD23 Monoclonal antibody (CL594-60208)

    IF Staining of human lymphoma using CL594-60208

    Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using CoraLite®594 CD23 antibody (CL594-60208, Clone: 4C7C9 ) at dilution of 1:200.

Tested Applications

Positive IF-P detected inhuman tonsillitis tissue, human lymphoma tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60208 targets CD23 in IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD23 fusion protein Ag0425
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Calculated Molecular Weight 321 aa, 36 kDa
GenBank Accession NumberBC064417
Gene Symbol CD23
Gene ID (NCBI) 2208
RRIDAB_2883442
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells. And the antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protocols

Product Specific Protocols
IF protocol for CL594 CD23 antibody CL594-60208Download protocol
Standard Protocols
Click here to view our Standard Protocols