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CoraLite®594-conjugated smooth muscle actin Polyclonal antibody

smooth muscle actin Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-14395

Synonyms

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, smooth muscle actin

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite®594-conjugated smooth muscle actin Polycl (CL594-14395)

    IF Staining of mouse heart using CL594-14395

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 smooth muscle actin antibody (CL594-14395) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using CoraLite®594-conjugated smooth muscle actin Polycl (CL594-14395)

    IF Staining of mouse small intestine using CL594-14395

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite®594 smooth muscle actin antibody (CL594-14395) at dilution of 1:200, CoraLite® Plus 488 ZG16 antibody (CL488-67389, Clone: 1A7B9, green).

Tested Applications

Positive IF-P detected inmouse heart tissue, mouse small intestine tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL594-14395 targets smooth muscle actin in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen smooth muscle actin fusion protein Ag5886
Full Name actin, alpha 2, smooth muscle, aorta
Calculated Molecular Weight 377 aa, 42 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC017554
Gene Symbol Alpha smooth muscle actin
Gene ID (NCBI) 59
RRIDAB_2919805
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.

Protocols

Product Specific Protocols
IF protocol for CL594 smooth muscle actin antibody CL594-14395Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Front Pharmacol

Pan-cancer analysis identifies NT5E as a novel prognostic biomarker on cancer-associated fibroblasts associated with unique tumor microenvironment

Authors - Xin-Miao Xue
View Article