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CoraLite® Plus 488-conjugated Nestin Polyclonal antibody

Nestin Polyclonal Antibody for IF/ICC, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19483

Synonyms

NES

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using CoraLite® Plus 488-conjugated Nestin Polyclonal an (CL488-19483)

    IF Staining of human kidney using CL488-19483

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 Nestin antibody (CL488-19483) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human dental pulp stem cells using CoraLite® Plus 488-conjugated Nestin Polyclonal an (CL488-19483)

    IF Staining of human dental pulp stem cells using CL488-19483

    Immunofluorescent analysis of fixed human dental pulp stem cells using CoraLite Plus 488 Nestin antibody (CL488-19483, in green) at dilution 1:250 and CoraLite 594 Iba1 antibody (CL594-10904, in red) at dilution 1:250. DAPI in blue. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

  • Immunofluorescence (IF) / fluorescent staining of human dental pulp stem cells using CoraLite® Plus 488-conjugated Nestin Polyclonal an (CL488-19483)

    IF Staining of human dental pulp stem cells using CL488-19483

    Immunofluorescent analysis of fixed human dental pulp stem cells using CoraLite Plus 488 Nestin antibody (CL488-19483, in green) at dilution 1:250 and CoraLite 594 Iba1 antibody (CL594-10904, in blue) at dilution 1:250. DAPI in red. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

  • Immunofluorescence (IF) / fluorescent staining of human dental pulp-derived neuronal spheroid using CoraLite® Plus 488-conjugated Nestin Polyclonal an (CL488-19483)

    IF Staining of human dental pulp-derived neuronal spheroid using CL488-19483

    Immunofluorescent analysis of fixed human dental pulp-derived neuronal spheroid using CoraLite Plus 488 Nestin antibody (CL488-19483, in green) and F-actin (in red). DAPI in blue. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

  • Immunofluorescence (IF) / fluorescent staining of human dental pulp-derived neuronal spheroids using CoraLite® Plus 488-conjugated Nestin Polyclonal an (CL488-19483)

    IF Staining of human dental pulp-derived neuronal spheroids using CL488-19483

    Immunofluorescent analysis of fixed human dental pulp-derived neuronal spheroids using CoraLite Plus 488 Nestin antibody (CL488-19483, in blue) and F-actin (in yellow). DAPI in purple. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

  • Immunofluorescence (IF) / fluorescent staining of human dental pulp stem cells using CoraLite® Plus 488-conjugated Nestin Polyclonal an (CL488-19483)

    IF Staining of human dental pulp stem cells using CL488-19483

    Immunofluorescent analysis of fixed human dental pulp stem cells using CoraLite Plus 488 Nestin antibody (CL488-19483, in green) at dilution 1:250 and CoraLite 594 Iba1 antibody (CL594-10904, in blue) at dilution 1:250. DAPI in red. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

Tested Applications

Positive IF-P detected inhuman kidney tissue
Positive IF/ICC detected inhuman dental pulp stem cells, human dental pulp-derived neuronal spheroids

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-19483 targets Nestin in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name nestin
Calculated Molecular Weight 177 kDa
Observed Molecular Weight 170-180 kDa
GenBank Accession NumberNM_006617
Gene Symbol Nestin
Gene ID (NCBI) 10763
RRIDAB_2919165
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Nestin antibody CL488-19483Download protocol
Standard Protocols
Click here to view our Standard Protocols