CoraLite® Plus 488-conjugated Nestin Polyclonal antibody

Nestin Polyclonal Antibody for IF/ICC, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19483

Synonyms

NES

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Validation Data Gallery

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  IF Staining of human kidney using CL488-19483

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 Nestin antibody (CL488-19483) at dilution of 1:200.

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  IF Staining of human dental pulp stem cells using CL488-19483

  Immunofluorescent analysis of fixed human dental pulp stem cells using CoraLite Plus 488 Nestin antibody (CL488-19483, in green) at dilution 1:250 and CoraLite 594 Iba1 antibody (CL594-10904, in red) at dilution 1:250. DAPI in blue. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

• 

  IF Staining of human dental pulp stem cells using CL488-19483

  Immunofluorescent analysis of fixed human dental pulp stem cells using CoraLite Plus 488 Nestin antibody (CL488-19483, in green) at dilution 1:250 and CoraLite 594 Iba1 antibody (CL594-10904, in blue) at dilution 1:250. DAPI in red. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

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  IF Staining of human dental pulp-derived neuronal spheroid using CL488-19483

  Immunofluorescent analysis of fixed human dental pulp-derived neuronal spheroid using CoraLite Plus 488 Nestin antibody (CL488-19483, in green) and F-actin (in red). DAPI in blue. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

• 

  IF Staining of human dental pulp-derived neuronal spheroids using CL488-19483

  Immunofluorescent analysis of fixed human dental pulp-derived neuronal spheroids using CoraLite Plus 488 Nestin antibody (CL488-19483, in blue) and F-actin (in yellow). DAPI in purple. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

• 

  IF Staining of human dental pulp stem cells using CL488-19483

  Immunofluorescent analysis of fixed human dental pulp stem cells using CoraLite Plus 488 Nestin antibody (CL488-19483, in green) at dilution 1:250 and CoraLite 594 Iba1 antibody (CL594-10904, in blue) at dilution 1:250. DAPI in red. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

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Tested Applications

• Positive IF-P detected in: human kidney tissue
• Positive IF/ICC detected in: human dental pulp stem cells, human dental pulp-derived neuronal spheroids

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-19483 targets Nestin in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nestin
• Calculated Molecular Weight: 177 kDa
• Observed Molecular Weight: 170-180 kDa
• GenBank Accession Number: NM_006617
• Gene Symbol: Nestin
• Gene ID (NCBI): 10763
• RRID: AB_2919165
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.