CoraLite®594-conjugated Nestin Polyclonal antibody
Nestin Polyclonal Antibody for IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF-P
Conjugate
CoraLite®594 Fluorescent Dye
Cat no : CL594-19483
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | rat brain tissue, human kidney tissue |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-19483 targets Nestin in IF-P applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | nestin |
Calculated Molecular Weight | 177 kDa |
Observed Molecular Weight | 170-180 kDa |
GenBank Accession Number | NM_006617 |
Gene Symbol | Nestin |
Gene ID (NCBI) | 10763 |
RRID | AB_2919852 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.
Protocols
Product Specific Protocols | |
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IF protocol for CL594 Nestin antibody CL594-19483 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |