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Anti-Mouse F4/80 Rabbit Recombinant Antibody

F4/80 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

241959G4

Cat no : 98236-1-RR

Synonyms

Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Balb/c mouse peritoneal macrophages using Anti-Mouse F4/80 Rabbit Recombinant Antibody (98236-1-RR)

    FC experiment of Balb/c mouse peritoneal macrophages using 98236-1-RR

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse F4/80 Rabbit Recombinant Antibody (98236-1-RR, Clone: 241959G4) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of Balb/c mouse peritoneal macrophages using Anti-Mouse F4/80 Rabbit Recombinant Antibody (98236-1-RR)

    FC experiment of Balb/c mouse peritoneal macrophages using 98236-1-RR

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse F4/80 Rabbit Recombinant Antibody (98236-1-RR, Clone: 241959G4) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Tested Applications

Positive FC detected inBalb/c mouse peritoneal macrophages

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98236-1-RR targets F4/80 in FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name EGF-like module containing, mucin-like, hormone receptor-like sequence 1
Calculated Molecular Weight102 kDa
GenBank Accession NumberNM_010130.4
Gene Symbol F4/80
Gene ID (NCBI) 13733
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

Protocols

Product Specific Protocols
FC protocol for F4/80 antibody 98236-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols