Anti-Mouse F4/80 Rabbit Recombinant Antibody, PBS Only

F4/80 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

241959G4

Cat no : 98236-1-PBS

Synonyms

Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1

---

Validation Data Gallery

• 

  FC experiment of Balb/c mouse peritoneal macrophages using 98236-1-RR (same clone as 98236-1-PBS)

  1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse F4/80 Rabbit Recombinant Antibody (98236-1-RR, Clone: 241959G4) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98236-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Balb/c mouse peritoneal macrophages using 98236-1-RR (same clone as 98236-1-PBS)

  1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse F4/80 Rabbit Recombinant Antibody (98236-1-RR, Clone: 241959G4) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98236-1-PBS in a different storage buffer formulation.

---

Product Information

98236-1-PBS targets F4/80 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• Calculated Molecular Weight: 102 kDa
• GenBank Accession Number: NM_010130.4
• Gene Symbol: F4/80
• Gene ID (NCBI): 13733
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.