CoraLite® Plus 647 Anti-Mouse F4/80 Rabbit Recombinant Antibody

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  FC experiment of mouse peritoneal macrophages using CL647-98236

  1x10^6 mouse peritoneal macrophages were surface stained with FITC Plus Anti-Mouse CD11b and 0.25 ug CoraLite® Plus 647 Anti-Mouse F4/80 Rabbit Recombinant Antibody (CL647-98236, Clone: 241959G4) or CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of mouse peritoneal macrophages using CL647-98236

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse F4/80 Rabbit Recombinant Antibody (CL647-98236, Clone:241959G4) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

F4/80 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-98236

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1

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Tested Applications

• Positive FC detected in: mouse peritoneal macrophages

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98236 targets F4/80 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• Calculated Molecular Weight: 102 kDa
• GenBank Accession Number: NM_010130.4
• Gene Symbol: F4/80
• Gene ID (NCBI): 13733
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q61549
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.