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Anti-Human IL-7 Rabbit Recombinant Antibody

IL-7 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

241000F3

Cat no : 98206-1-RR

Synonyms

IL7, Interleukin-7, interleukin 7, IL 7, 241000F3

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Human IL-7 Rabbit Recombinant Antibody (98206-1-RR)

    FC experiment of Transfected HEK-293T using 98206-1-RR

    1x10^6 HEK-293T cells or IL-7 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Human IL-7 Rabbit Recombinant Antibody (98206-1-RR, Clone:241000F3) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Human IL-7 Rabbit Recombinant Antibody (98206-1-RR)

    FC experiment of Transfected HEK-293T using 98206-1-RR

    1x10^6 HEK-293T cells (left) or IL-7 transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Human IL-7 Rabbit Recombinant Antibody (98206-1-RR, Clone:241000F3) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98206-1-RR

    Affinity and Kinetic Characterization of 98206-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98206-1-RR against Human IL-7 were performed. The affinity constant is 8.55 nM.

Tested Applications

Positive FC (Intra) detected inTransfected HEK-293T cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98206-1-RR targets IL-7 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name interleukin 7
Calculated Molecular Weight20 kDa
GenBank Accession NumberNM_000880.4
Gene Symbol IL-7
Gene ID (NCBI) 3574
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

Interleukin-7 (IL-7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL-7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL-7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration. (PMID: 29663382; 29655570; 29449560)

Protocols

Product Specific Protocols
FC protocol for IL-7 antibody 98206-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols