Anti-Human IL-7 Rabbit Recombinant Antibody, PBS Only

IL-7 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

241000F3

Cat no : 98206-1-PBS

Synonyms

IL7, Interleukin-7, interleukin 7, IL 7, 241000F3

---

Validation Data Gallery

• 

  FC experiment of Transfected HEK-293T using 98206-1-RR (same clone as 98206-1-PBS)

  1x10^6 HEK-293T cells or IL-7 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Human IL-7 Rabbit Recombinant Antibody (98206-1-RR, Clone:241000F3) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98206-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Transfected HEK-293T using 98206-1-RR (same clone as 98206-1-PBS)

  1x10^6 HEK-293T cells (left) or IL-7 transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Human IL-7 Rabbit Recombinant Antibody (98206-1-RR, Clone:241000F3) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98206-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98206-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98206-1-RR against Human IL-7 were performed. The affinity constant is 8.55 nM.

---

Product Information

98206-1-PBS targets IL-7 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 7
• Calculated Molecular Weight: 20 kDa
• GenBank Accession Number: NM_000880.4
• Gene Symbol: IL-7
• Gene ID (NCBI): 3574
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-7 (IL-7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL-7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL-7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration. (PMID: 29663382; 29655570; 29449560)