Biotin Anti-Human CD2 Rabbit Recombinant Antibody

CD2 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Biotin

CloneNo.

241222E2

Cat no : Biotin-98134

Synonyms

LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor, CD2 molecule

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Validation Data Gallery

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  FC experiment of human PBMCs using Biotin-98134

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD2 Rabbit Recombinant Antibody (Biotin-98134, Clone: 241222E2) and CoraLite®488-conjugated streptavidin, or unstained. Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using Biotin-98134

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD2 Rabbit Recombinant Antibody (Biotin-98134, Clone: 241222E2) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of Biotin-98134 is CD2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD2 molecule
• Calculated Molecular Weight: 351 aa, 39 kDa
• GenBank Accession Number: BC033583
• Gene Symbol: CD2
• Gene ID (NCBI): 914
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.