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Anti-Human CD72 Rabbit Recombinant Antibody

CD72 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241300C1

Cat no : 98090-1-RR

Synonyms

LYB2, Lyb 2, CD72b, CD72 molecule, B-cell differentiation antigen CD72

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD72 Rabbit Recombinant Antibody (98090-1-RR)

    FC experiment of human PBMCs using 98090-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD72 Rabbit Recombinant Antibody (98090-1-RR, Clone:241300C1) , or Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD72 Rabbit Recombinant Antibody (98090-1-RR)

    FC experiment of human PBMCs using 98090-1-RR

    1x10^6 human PBMCs were stained with 0.25 ug Anti-Human CD72 Rabbit Recombinant Antibody (98090-1-RR, Clone:241300C1)(red) , or Isotype Control(blue) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD72 Rabbit Recombinant Antibody (98090-1-RR)

    FC experiment of human PBMCs using 98090-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD72 Rabbit Recombinant Antibody (98090-1-RR, Clone:241300C1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug Isotype Control, and 0.25 ug CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed.Lymphocyte were gated.

  • Affinity and Kinetic Characterization of 98090-1-RR

    Affinity and Kinetic Characterization of 98090-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98090-1-RR against Human CD72 were performed. The affinity constant is 10.7 nM.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98090-1-RR targets CD72 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name CD72 molecule
Calculated Molecular Weight 359 aa, 40 kDa
GenBank Accession NumberBC030227
Gene Symbol CD72
Gene ID (NCBI) 971
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).

Protocols

Product Specific Protocols
FC protocol for CD72 antibody 98090-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols