Anti-Human CD72 Rabbit Recombinant Antibody, PBS Only

CD72 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241300C1

Cat no : 98090-1-PBS

Synonyms

LYB2, Lyb 2, CD72b, CD72 molecule, B-cell differentiation antigen CD72

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Validation Data Gallery

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  FC experiment of human PBMCs using 98090-1-RR (same clone as 98090-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD72 Rabbit Recombinant Antibody (98090-1-RR, Clone:241300C1) , or Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. This data was developed using the same antibody clone with 98090-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98090-1-RR (same clone as 98090-1-PBS)

  1x10^6 human PBMCs were stained with 0.25 ug Anti-Human CD72 Rabbit Recombinant Antibody (98090-1-RR, Clone:241300C1)(red) , or Isotype Control(blue) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).Cells were not fixed. This data was developed using the same antibody clone with 98090-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98090-1-RR (same clone as 98090-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD72 Rabbit Recombinant Antibody (98090-1-RR, Clone:241300C1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug Isotype Control, and 0.25 ug CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed.Lymphocyte were gated. This data was developed using the same antibody clone with 98090-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98090-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98090-1-RR against Human CD72 were performed. The affinity constant is 10.7 nM.

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Product Information

98090-1-PBS targets CD72 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: CD72 molecule
• Calculated Molecular Weight: 359 aa, 40 kDa
• GenBank Accession Number: BC030227
• Gene Symbol: CD72
• Gene ID (NCBI): 971
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).