FITC Plus Anti-Human CD72 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using FITC-98090

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD72 Rabbit RecAb (FITC-98090, Clone: 241300C1) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-98090

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD72 Rabbit RecAb (FITC-98090, Clone:241300C1) (red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). Cells were not fixed. Lymphocytes were gated.

CD72 Uni-rAb^TM Recombinant Antibody for FC

Cat No. FITC-98090

Clone No.241300C1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

LYB2, Lyb 2, CD72b, CD72 molecule, B-cell differentiation antigen CD72

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-98090 targets CD72 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: CD72 molecule
• Calculated Molecular Weight: 359 aa, 40 kDa
• GenBank Accession Number: BC030227
• Gene Symbol: CD72
• Gene ID (NCBI): 971
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21854
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).