Filter: AllSandwich ELISASample testIF/ICCFC (Intra)

Sandwich ELISA standard curve of MP01960-1
Sandwich ELISA standard curve of MP01960-1, Human Filamin B Recombinant Matched Antibody Pair - PBS only. 85202-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY00901. 85202-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.
Sample test of MP01960-1
The mean Filamin B concentration was determined to be 4.9 ng/mL in A549 cell extract based on a 2.6 mg/mL extract load and 5.5 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load.
Sample test of MP01960-1
Serum of six individual healthy human donors was measured. The Filamin B concentration of detected samples was determined to be 1.1 ng/mL with a range of 0.4-1.9 ng/mL.
IF Staining of HeLa using 85202-1-RR (same clone as 85202-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Filamin B antibody (85202-1-RR, Clone: 242275B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85202-1-PBS in a different storage buffer formulation.
IF Staining of A549 using 85202-1-RR (same clone as 85202-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed A549 cells using Filamin B antibody (85202-1-RR, Clone: 242275B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85202-1-PBS in a different storage buffer formulation.
FC experiment of HEK-293T using 85202-1-RR (same clone as 85202-1-PBS)
1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Filamin B Recombinant antibody (85202-1-RR, Clone:242275B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85202-1-PBS in a different storage buffer formulation.
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1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Filamin B Recombinant antibody (85202-1-RR, Clone:242275B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85202-1-PBS in a different storage buffer formulation.

Filamin B Recombinant antibody, PBS Only (Detector)

Filamin B {ptg:Brand} Recombinant Antibody for IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85202-1-PBS

Clone No.242275B7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

FLNB, Filamin-3, Beta-filamin, Actin-binding-like protein, ABP-280 homolog

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

85202-1-PBS targets Filamin B as part of a matched antibody pair:

MP01960-1: 85202-2-PBS capture and 85202-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	filamin B, beta (actin binding protein 278)
Calculated Molecular Weight	278 kDa
GenBank Accession Number	NM_001457
Gene Symbol	FLNB
Gene ID (NCBI)	2317
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O75369
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

FLNB, also named as FLN1L, FLN3, TABP, TAP and Fh1, belongs to the filamin family. FLNB connects cell membrane constituents to the actin cytoskeleton. It may promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. It is an anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA, FLNB may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis.