Filamin B Recombinant antibody

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  IF Staining of HeLa using 85202-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Filamin B antibody (85202-1-RR, Clone: 242275B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A549 using 85202-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Filamin B antibody (85202-1-RR, Clone: 242275B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HEK-293T using 85202-1-RR

  1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Filamin B Recombinant antibody (85202-1-RR, Clone:242275B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Filamin B {ptg:Brand} Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Cat No. 85202-1-RR

Clone No.242275B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

FLNB, Filamin-3, Beta-filamin, Actin-binding-like protein, ABP-280 homolog

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Tested Applications

• Positive IF/ICC detected in: HeLa cells, A549 cells
• Positive FC (Intra) detected in: HEK-293T cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85202-1-RR targets Filamin B in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: filamin B, beta (actin binding protein 278)
• Calculated Molecular Weight: 278 kDa
• GenBank Accession Number: NM_001457
• Gene Symbol: FLNB
• Gene ID (NCBI): 2317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75369
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FLNB, also named as FLN1L, FLN3, TABP, TAP and Fh1, belongs to the filamin family. FLNB connects cell membrane constituents to the actin cytoskeleton. It may promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. It is an anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA, FLNB may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis.